The Image Formation Process in Differential Interference Contrast (DIC) Microscopy: A Ray Tracer Model
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Differential interference contrast x-ray microscopy with submicron resolution
Progress in lithography and nanofabrication @E. Di Fabrizio et al., Nature ~London! 401, 895 ~1999!# has made it possible to apply differential interference contrast ~DIC! in x-ray microscopy using an original x-ray doublet lens based on two specially developed zone plates. Switching from bright-field imaging ~absorption contrast! to x-ray DIC, we observe, similar to visible-light microscopy, a...
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We present the defocused differential interference contrast (DIC) imaging of gold nanorods. We found that the scattered light and the defocus aberration play an important role in the formation of orientation-dependent DIC image patterns of a gold nanorod. Interestingly, the scattered light from a gold nanorod aligned closer to the polarization directions enables us to directly resolve its spati...
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X-ray imaging in differential interference contrast (DIC) with submicrometer optical resolution was performed by using a twin zone plate (TZP) setup generating focal spots closely spaced within the TZP spatial resolution of 160 nm. Optical path differences introduced by the sample are recorded by a CCD camera in a standard full-field imaging and by an aperture photodiode in a standard scanning ...
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Differential interference contrast (DIC) microscopy is a powerful visualization tool used to study live biological cells. Its use, however, has been limited to qualitative observations. The inherent nonlinear relationship between the object properties and the image intensity makes quantitative analysis difficult. Toward quantitatively measuring optical properties of objects from DIC images, we ...
متن کاملTheoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy.
Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generat...
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تاریخ انتشار 2006